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细胞名称

C6大鼠胶质瘤细胞

描述

胶质细胞株C6是由Benda等用N-nitrosomethylurea诱导的大鼠胶质瘤克隆，并经过一系列的体外培养和动物传代交替后建成的。 当细胞从低密度生长到满瓶时，S-100产量增加10倍。

动物种别

大鼠

性别

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组织来源

胶质瘤，脑，胶质细胞

形态

成纤维细胞

培养基和添加剂

F12K培养基(SIGMA,货号N3520，添加NaHCO3 2.5g/L)，82.5%；马血清，15%；优质胎牛血清，2.5%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。

REFERENCE

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